Hemolysis primarily elevates which LD isoenzymes, potentially causing a falsely high result?

Hemolysis primarily elevates which LD isoenzymes, potentially causing a falsely high result?

• A. LD1 and LD2
• B. LD3
• C. LD4
• D. LD5

Answer: (A)

Hemolysis releases enzymes from red blood cells, and the red cells are rich in LDH-1 and LDH-2. When the cells rupture, these isoenzymes flood the serum, lifting the total LD measurement. The other LD isoenzymes (LD-3, LD-4, LD-5) are not as abundant in RBCs, so they contribute less to the increase. So, the isoenzymes most affected by hemolysis are LD1 and LD2, leading to a falsely high LD result. This interference is why hemolyzed samples are often flagged or rejected in LD testing.

Hemolysis releases enzymes from red blood cells, and the red cells are rich in LDH-1 and LDH-2. When the cells rupture, these isoenzymes flood the serum, lifting the total LD measurement. The other LD isoenzymes (LD-3, LD-4, LD-5) are not as abundant in RBCs, so they contribute less to the increase. So, the isoenzymes most affected by hemolysis are LD1 and LD2, leading to a falsely high LD result. This interference is why hemolyzed samples are often flagged or rejected in LD testing.