The chromophore measured in the Jendrassik-Grof bilirubin assay is:

The chromophore measured in the Jendrassik-Grof bilirubin assay is:

• A. Azobilirubin
• B. Bilirubin glucuronide
• C. Urobilin
• D. Urobilinogen

Answer: (A)

In this assay, bilirubin is measured by a diazo reaction that converts bilirubin into an azo dye called azobilirubin. The diazotized sulfanilic acid reacts with bilirubin in acid medium to form azobilirubin, which has a strong visible absorbance and is quantified spectrophotometrically. This azo dye is the actual chromophore detected, not bilirubin glucuronide, urobilin, or urobilinogen, which do not form the colored azo compound in this method. Hence, azobilirubin is the measured chromophore in the Jendrassik-Grof bilirubin assay.

In this assay, bilirubin is measured by a diazo reaction that converts bilirubin into an azo dye called azobilirubin. The diazotized sulfanilic acid reacts with bilirubin in acid medium to form azobilirubin, which has a strong visible absorbance and is quantified spectrophotometrically. This azo dye is the actual chromophore detected, not bilirubin glucuronide, urobilin, or urobilinogen, which do not form the colored azo compound in this method. Hence, azobilirubin is the measured chromophore in the Jendrassik-Grof bilirubin assay.